12. 11. Clostridium tetani is a slender, obligate anaerobic bacillus measuring 0.5–1.7 µm by 2.1–18.1 µm. A: B: C: IV. Source Shape: Spore-forming Rod. With this staining procedure, the vegetative bacteria stain blue and the endospores are colorless. Experiment (dry lab) Record the cell shape. Tetanus is contracted through contact between spores of C. tetani and an … Clostridium tetani is an anaerobic pathogenic bacterium that is primarily found in soil and animal intestinal tracts. Clostridium tetani is a gram positive, spore-forming, obligate anaerobic, rod-shaped bacterium of the phylum firmicutes. A single, terminal spore is present at the end of each rod, giving a "Tennis Racket" like appearance: Transmission; C. tetani spores are common in the soil and may exist on sharp objects on the ground like rusty nails. Most species grow only in the complete absence of oxygen. Shapes and Arrangements. 13). Habitat. Dormant cells are highly resistant to heat, desiccation, and toxic chemicals It is capsulated, non-motile and non-flagellated pathogenic bacteria. Clostridium tetani is the etiological agent of tetanus, Clostridium botulinum is the etiological agent of botulism, and Clostridium perfringens is one of the etiological agent of gas gangrene. Diseases. Clostridium, genus of rod-shaped, usually gram-positive bacteria, members of which are found in soil, water, and the intestinal tracts of humans and other animals. Note the "tennis racquet" appearance of the endospore-containing Clostridium. As characteristic of all bacteria, C. tetani bacteria are single-celled and do not contain any membrane-bound organelles, such as a nucleus. Produce round, terminal endospores that give the bacterium a "tennis-racquet" appearance. CHARACTERISTICS: Clostridium tetani is a motile, anaerobic, spore forming bacteria (terminal spores with drum stick appearance) Footnote 1, Footnote 2. Size is about 3-8 µm X 0.4-1.2 µm. The muscle stiffness usually involves the jaw (lockjaw) and neck and then becomes generalized. ; It is pleomorphic with straight or curved rods. Found in nature as spores in soil or parasitising the gastrointestinal tracts of animals, these bacteria cause serious toxicity in humans. C. tetani's appearance on gram stain is said to resemble tennis rackets or drumsticks. Endospore stain of Clostridium botulinum (see Fig. Clostridium perfringens is large rectangular gram-positive bacilli with rounded or truncated ends. Clostridium tetani is a moderately-sized gram-positive, endospore-producing bacillus. An obligate anaerobe. Clostridium tetani . Clostridium tetani. 16 Although classified as a Gram-positive organism, it may stain variably, especially in tissue or older cultures. Organism. Colonizes the intestinal tract in humans and animals. Vegetative cells are rod shaped, pleomorphic, and occur in pairs or short chains Footnote 1. 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